Fig 1: PPI network of DEGs, hub genes selection, and survival analysis of four key genes.(A) PPI network of 104 DEGs, the representation of nodes and edges are protein and the interaction of the proteins. (B) Seventeen hub genes of DEGs were obtained with module analysis using the MCODE app. (P < 0.01, KIRC: kidney renal clear cell carcinoma). (C) Survival analysis of PCOLCE. (D) Survival analysis of P4HB. (E) Survival analysis of COL6A2. (F) Survival analysis of COL6A3.
Fig 2: Expression of key genes in primary ccRCC and the corresponding metastatic ccRCC.(A) the qRT-PCR analysis showed the expression of PCOLCE, P4HB, COL6A2 and COL6A3 in primary ccRCC cell line Caki-2 and its corresponding metastatic ccRCC cell line Caki-1. (***P < 0.001, ****P < 0.0001) (B) Representative IHC staining images of PCOLCE, P4HB, COL6A2 and COL6A3 in human primary ccRCC and corresponding metastatic ccRCC (22 paired cases) are on the left, and the quantitative results on the right. 200x. (***P < 0.001, ****P < 0.0001).
Supplier Page from Abcam for Anti-PCOLCE antibody